Search: cavalier/info-lgs@agrotis.it – Page 4

Need a new search?

If you didn't find what you were looking for, try a new search!

Détermination du profil ADN équin

This product contains a DNA profile for a single horse.
A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.
In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

DNA Profile Cat – SNP (ISAG 2020)

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

Two types of markers are available for DNA profiling and parentage verification: STR (microsatellite) markers and SNP markers. These markers rely on different technologies, and their results are not interchangeable. If you’re unsure about the difference between these marker sets, please reach out to us before ordering a test to determine which marker type is necessary for performing the DNA profile or parentage verification.

DNA Profile Cat – STR + SNP (ISAG 2020)

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

Two types of markers are available for DNA profiling and parentage verification: STR (microsatellite) markers and SNP markers. These markers rely on different technologies, and their results are not interchangeable. If you’re unsure about the difference between these marker sets, please reach out to us before ordering a test to determine which marker type is necessary for performing the DNA profile or parentage verification.

DNA Profile Dog – ISAG 2006 (STR) + ISAG 2020 (SNP)

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

Two types of markers are available for DNA profiling and parentage verification: STR (microsatellite) markers and SNP markers. These markers rely on different technologies, and their results are not interchangeable. If you’re unsure about the difference between these marker sets, please reach out to us before ordering a test to determine which marker type is necessary for performing the DNA profile or parentage verification.

DNA Profile Dog – ISAG 2020 (SNP)

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

Two types of markers are available for DNA profiling and parentage verification: STR (microsatellite) markers and SNP markers. These markers rely on different technologies, and their results are not interchangeable. If you’re unsure about the difference between these marker sets, please reach out to us before ordering a test to determine which marker type is necessary for performing the DNA profile or parentage verification.

DNA Profile – Llama / Alpaca

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

DNA Profile Dog – ASCA (SNP)

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

DNA Profile Dog – ISAG 2006 (STR)

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

Two types of markers are available for DNA profiling and parentage verification: STR (microsatellite) markers and SNP markers. These markers rely on different technologies, and their results are not interchangeable. If you’re unsure about the difference between these marker sets, please reach out to us before ordering a test to determine which marker type is necessary for performing the DNA profile or parentage verification.

DNA Profile Cat – STR

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

Two types of markers are available for DNA profiling and parentage verification: STR (microsatellite) markers and SNP markers. These markers rely on different technologies, and their results are not interchangeable. If you’re unsure about the difference between these marker sets, please reach out to us before ordering a test to determine which marker type is necessary for performing the DNA profile or parentage verification.

CombiBreed Kooikerhondje – Fit2Breed

This Combination Pack is designed to provide you with vital insights into your dog’s genetic health and diversity and includes DNA tests for important diseases. In addition, we also calculate the Coefficient of Inbreeding (COI) and the percentage of Heterozygosity of your dog’s DNA. The COI shows the degree of inbreeding of your dog, whereas the Heterozygosity percentage is a measure of your dog’s individual genetic diversity.

By ordering this combination package, you authorize us to share the 35000 SNP data (your dog’s genetic results) linked to the chip number with Utrecht University, our research partner. This data will be used to update your profile in the Fit2Breed database and to enable Utrecht University to conduct anonymized scientific research. The data transfer occurs automatically, and no status updates will be provided.

For more information about the collaboration between Fit2Breed and the Vereniging Het Nederlandse Kooikerhondje, please visit their <a href=”https://www.kooikerhondje.nl/fokkerij/fit2breed/” target=”_new”>website</a>.
To order this package, we recommend using <a href=”https://survey.uu.nl/jfe/form/SV_5bYsJ3YJIdgj4O2″ target=”_new”>this link</a> to Utrecht University. There, you can easily provide the required information for Fit2Breed and complete the ordering process. Please note that the procedure must be carried out by a qualified vet. A Witness Sampling Statement is therefore required when ordering this CombiBreed package.

Information about individual tests in this package is available in the section ‘Included Tests’ on this page. We accept samples from animals of any age. Normally, the turnaround time of tests performed at our own facilities is 10 working days after receipt of the sample. For outsourced tests, so-called “External lab”, or “External Patent lab”, the turnaround time is at least 20 working days after receipt of your sample. Please note that the mentioned 20 working days is an estimate, as the shipping time to these external laboratories or patent facilities may vary due to unexpected delays.

Some tests included are performed by an external laboratory. CombiBreed takes care of the mediation between you as a customer and the external laboratory. In these cases, CombiBreed cannot be held liable for the behaviour of the client and/or contractor.

DNA Profile – Pigeon

This product contains a DNA profile for a single animal.

A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

In addition to parentage verification, a DNA profile can be employed to confirm the identity of an individual. The reliability of such an analysis is extremely high, as it necessitates an exact match of all genetic information between two samples.

A propos de nous

En 1986, le vétérinaire Dr. Hein van Haeringen a fondé une société de typage sanguin. La société a été fondée pour fournir à la fois des services de recherche et de diagnostic pour les animaux. À cette époque, le Dr. van Haeringen a remarqué que les animaux parents mouraient de la même maladie que leur progéniture. Ces maladies sont appelées maladies héréditaires, qui sont des maladies qui sont transmises des parents à leur progéniture. La demande de justification scientifique devient importante afin de sauver la vie de ces animaux.

Lorsque la possibilité de prévenir leurs animaux de maladies héréditaires est arrivée, il est devenu une forte demande pour ce type de tests génétiques. Sauver la santé animale était nécessaire pour survivre. La fondation, le Dr. Van Haeringen Laboratorium (VHL) a proposé un groupe sanguin chez plusieurs espèces animales. De cette façon, les éleveurs ont eu la possibilité d’optimiser leurs choix d’élevage et d’éviter à leurs animaux des problèmes de santé.

Au cours des années 1990 et 2000, le génotypage est devenu plus rentable et donc commercialement plus attrayant pour être largement utilisé. De nouvelles bases de données contenaient d’énormes quantités de données, permettant de combiner les génotypes pour l’analyse du pedigree, le génotypage des mutations causales et les calculations des valeurs de sélection génétique. Ces valeurs ont fourni de nouvelles perspectives pour l’industrie de l’élevage et ont conduit par la suite à l’amélioration d’une sélection plus précise.

Le Dr. Van Haeringen Laboratorium est devenu largement connu pour son expertise en génétique et en génomique. La reconnaissance a été acquise à la suite de bons services génomiques constants. De nouvelles méthodes étaient prêtes à être mises en œuvre à tout moment, s’adaptant à l’augmentation des volumes d’échantillons au fil des ans. Au fur et à mesure que le Dr. Van Haeringen Laboratorium est devenu plus connu à l’échelle internationale, il a été décidé de continuer sous la dénomination sociale VHLGenetics. De là, deux nouveaux sites (Belgique et Allemagne) ont été ouverts afin de servir nos clients sur le globe plus rapidement et dans leur propre langue. En outre, VHL a commencé à collaborer avec plusieurs distributeurs. Ces collaborations ont été initiées pour combiner l’expertise de ces entreprises et conduire consécutivement à l’augmentation de la santé et du bien-être des animaux. Royal GD est devenu l’un de nos actionnaires en 2009. Royal GD became one of our shareholders in 2009. Fin 2019, GD est devenue notre société mère et a repris l’entreprise de la famille Van Haeringen. En unissant nos forces, nous nous efforçons d’obtenir la santé la plus optimale de votre animal de compagnie.

En juillet 2021, les sociétés BioBank et VHLGenetics ont démarré ensemble le nouveau laboratoire de génotypage ScandiGen Lab à Hamar, en Norvège. Pour les deux sociétés, il s’agissait d’une étape logique après que BioBank soit devenue distributeur de VHLGenetics en 2017. La coopération réussie a conduit à la création de ScandiGen Lab qui unit les connaissances et les forces des deux sociétés.

Au cours des deux dernières années, la sensibilisation à la santé et au bien-être des animaux est devenue de plus en plus importante dans le monde. Cette conscience s’est développée plus rapidement ces dernières années. Un mode de vie sain basé sur le profil génétique de votre animal est le nouvel avenir. C’est pourquoi nous voulons être prêts pour nos clients et nous aimons réfléchir avec vous. Par conséquent, nous travaillons dur pour optimiser notre service et nos technologies. Nous ne vous donnons plus seulement des données en tant qu’éleveur, vétérinaire ou propriétaire d’animal de compagnie, mais nous devenons votre partenaire de connaissances et votre guide de mode de vie animal. Le partenaire scientifique qui vous aide à prendre les meilleures décisions pour un monde avec le moins de maladies héréditaires possible pour nos animaux.

À ce jour, nous avons trois emplacements où nous effectuons nos tests ADN. Ces sites sont basés aux Pays-Bas, en Belgique et en Allemagne, avec le siège social à Wageningen (Pays-Bas). Nous sommes représentés dans différents pays européens par nos propres bureaux de vente et notre réseau de distribution.

Les tests ADN sont effectués sous diverses accréditations, certifications et adhésions à des organisations telles que ICAR et ISAG. Grâce à la coopération avec nos distributeurs et d’autres partenaires solides, nous offrons nos tests ADN dans le monde entier, avec un excellent service local.

99

Euro

Excl. BTW

Få svar på hvilke raser som er i stamtreet til hunden din

Parentage Verification using DNA

Complexity of genetic material

The body of an organism consists of a large number of cells, which contain a full and complete set of genetic material. Genetic information is present in the nucleus of a cell. The genetic information is stored in chromosomes, which are translated by the body in useful data (proteins). This happens constantly in all cells. The general code is called DNA.

Chromosomes exist of long DNA-strains which are wound around each other very tightly. When a chromosome is studied in detail, it is possible to look at the composition of DNA in the form of A, T, G, or C. These A, T, G and C are the building blocks from which the DNA is constructed. Sometimes stretches of repeats are present (e.g. CACACA) – such stretches are indicated as microsatellites (also known as STRs). Other variation such as G/A or C/G is indicated as Single Nucleotide Polymorphism (SNP). The order and composition of DNA are the basis for all kinds of applications.

For the typing of the composition of genetic characteristics it is possible to use hairs, feathers – to be drawn with roots –, blood, milk, tissue etcetera. The usability of sample material depends on the test which is carried out. The use of ‘fresh’ material provides the best result.


Techniques

Genetic variation can be visualized with a number of different techniques. Frequently a technique is used, where DNA is multiplied (PCR). DNA can be made visible through three steps:

  • DNA-extraction, where the cells are broken into small pieces. The DNA is present in an aqueous solution, which is necessary to enable a successful PCR-reaction,
  • Selective multiplying of DNA, where PCR is used to multiply small specific pieces,
  • Analysis of DNA on a machine, with which DNA is visualized. For that purpose, fluorescence is incorporated during PCR.

The final result of these steps results generally in the detection of the variation in STRs or SNPs. By examining a number of STRs or SNPs, a genetic bar code is generated. This bar code can be used for a number of different tests, among which are lineage, identity of samples, etcetera. In a number of examples these applications are described below.

Coat Colour Silver Dilution (MCOA)

Silver coat colour, known as Silver Dapple, is a “dilution” or lightening of the black pigment in the horse’s hair, resulting in a silvery-grey or even white coat colour. It is a dominant trait, caused by a mutation to the PMEL gene, and it is found in multiple breeds. It should not be confused with G-Locus Grey, which causes gradual but permanent lightening of the horse’s coat.

Horses carrying the Silver mutation are also noted to be at risk for Multiple Congenital Ocular Anomalies (MCOA, also known as Anterior Segment Dysgenesis and as Congenital Aniridia). MCOA can cause a range of eye defects.

Panda White Spotting

A mutation in the KIT-gene is associated with a white spotting pattern in German Shepherd Dogs, this pattern is  also called Panda White Spotting. The mutation is very recent, it appeared spontaneously in a female born in 2000. The gene for white-spotting is known as the S-locus (MITF-Gene), however this mutation in the German Shepherd dogs is in a different gene then the mutation causing white spotting in other dog breeds. The mutation causes white markings on the face, limbs, belly, neck, and tip of the tail, with the white being concentrated toward the front of the dog, similar to the irish spotting pattern. The amount of white can vary from dog to dog. The mutation that causes the Panda White pattern in German Shepherd dogs is in homozygous state (two copies of the mutation) considered embryonic lethal as no live dogs with the pattern and with two copies of the mutation have been observed. This means that pups that are homozygous for the Panda mutation do not develop in the uterus and are reabsorbed very early in the development process. Dogs that are heterozygous (one copy of the mutation) do not have any health defects associated with the Panda pattern. The Coat Colour Panda White Spotting test (H354) tests for the genetic status of the KIT-gene. This gene has two variants (alleles), P and N. The allele P is dominant. One copy of the P allele results in dogs with the Panda white pattern. Two copies of the P allele result in early embryonic death. The allele N does have no effect on the coat colour.

The Coat Colour Panda White Spotting test encloses the following results.

KIT-gene

Coat Colour

N/N

No Panda White spotting unless modified by other colour modifying factors, only allele N will be passed on to an offspring

N/P

Panda White spotting, either allele N or P will be passed on to an offspring

CLAD (Canine Leukocyte Adhesion Deficiency) Type I – Irish Setter

Canine Leukocyte Adhesion Deficiency (CLAD) is a fatal immunodeficiency disease. It is a genetic condition caused by specific mutations in genes crucial for platelet and blood cell functions. These mutations result in abnormal blood clotting and immune system responses in affected dogs. This variant of the disease, CLAD Type I, is caused by a recessive mutation in the ITGB2 gene, which encodes the leukocyte integrin beta-2 subunit (CD18). The disease is found in the Irish Setter.

Parentage Verification using single nucleotide polymorphisms (SNPS)

As with STRs, the basis is the same. However, more genetic markers are tested because the information content per markers is lower for SNPs.

The genetic variation which is present in an animal, originates from both parents. Half of the variation is originating from the father, whereas the other half comes from the mother.

For parentage verification, typically 200 up to 400 genetic characteristics are visualized. In this process the actual genetic composition (A,C,G, or T) is being measured. The composition/variant  in an offspring must correspond to the composition/variant in the mother and father that were provided for comparison. In two examples, it is shown how the basic rules are applied in parentage verification.

Marker

Offspring

Mother

Father

SNP01

AT

AA

TT

SNP02

GC

GC

CC

SNP03

TT

CT

TT

SNP04

AC

AC

AC

SNP05

CC

CC

CT

SNP06

CT

CC

CT

In the above table an example is provided of a correct parentage. In this table, the DNA is shown of three individuals: an offspring (left column), a potential mother (middle column), and a potential father (right columns). In each line one variant is shown. In this case all variants in the offspring are present in the parents: the parentage is correct.

Marker

Offspring

Mother

Father

SNP01

AA

AA

TT

SNP02

GC

GC

CC

SNP03

CC

CT

TT

SNP04

AC

AC

AC

SNP05

CC

CC

CT

SNP06

CT

CC

CT

In the second table an example is provided of an incorrect parentage. In this table, the DNA is shown of three individuals: an offspring (left column), a potential mother (middle column), and a potential father (right columns). In each line one variant is shown. In this case several variants are present in the offspring which are not are present in the parents: the parentage is not correct.

When 200 up to 400 different genetic fragments are checked, the chance that an incorrect parentage is not detected becomes very small. The genetic fragments which are used for parentage verification and identification provide no information on properties such as color and quality of an animal, plant or human, since the fragments are non-coding.

CombiBreed SNP Data Fit2Breed

By ordering this product, you authorize us to share the 35000 SNP data (your dog’s genetic results) linked to the chip number with Utrecht University, our research partner. This data will be used to update your profile in the Fit2Breed database and to enable Utrecht University to conduct anonymized scientific research. The data transfer occurs automatically, and no status updates will be provided.

For more information about the collaboration between Fit2Breed and the Vereniging Het Nederlandse Kooikerhondje, please visit their website.
To order this package, we recommend using this link to Utrecht University. There, you can easily provide the required information for Fit2Breed and complete the ordering process.

Paternity Verification Dog – ASCA (SNP)

This product includes paternity verification and DNA profiling of the offspring. If the DNA profile of the (expected) father is unavailable, a separate DNA profile needs to be ordered.
A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

If the DNA profile of the parent(s) is performed in a laboratory other than VHL / CTG / VHP, it can occur the profile of the offspring can’t be compared with the profiles of the parents. The reason can be the testing of other hereditary characteristics at the other laboratory.

Maternity Verification Dog – ASCA (SNP)

This product includes maternity verification and DNA profiling of the offspring. If the DNA profile of the (expected) mother is unavailable, a separate DNA profile needs to be ordered.
A DNA profile is established using DNA markers. The profile from each sample is stored in a database and can be represented as a barcode, which is unique to each individual. This DNA profile serves the purpose of parentage verification, involving a comparison of the genetic information present in an offspring with that of the potential parents. For accurate parentage verification, all genetic information in the offspring must be traceable to the combination of the dam and the sire. In the majority of cases, the reliability of this analysis exceeds 99.5 percent.

If the DNA profile of the parent(s) is performed in a laboratory other than VHL / CTG / VHP, it can occur the profile of the offspring can’t be compared with the profiles of the parents. The reason can be the testing of other hereditary characteristics at the other laboratory.

Go to Top